|
KMID : 0043320030260121087
|
|
Archives of Pharmacal Research
2003 Volume.26 No. 12 p.1087 ~ p.1095
|
|
|
Immunomodulatory Activity of Betulinic Acid by Producing Pro-Inflammatory Cytokines and Activation of Macrophages
|
|
Yun Yun-Ha
Han Shin-Ha
Park Eun-Jung
Yim Don-Sool
Lee Sook-Yeon
Lee Chong-Kil
Cho Kyung-Hae
Kim Kyung-Jae
|
|
|
Abstract
|
|
|
|
Betulinic acid (BA), a pentacyclic triterpene isolated from Lycopus lucidus, has been reported to be a selective inducer of apoptosis in various human cancer and shown anti-inflammatory and immunomodulatory properties. We postulated that BA modulates the immunomodulatory properties at least two groups of protein mediators of inflammation, interlukin-1 (IL-1) and the tumor necrosis factor- (TNF-) on the basis of the critical role of the monocytes and tissue macrophages in inflammatory and immune responses. TNF- and IL-1 were produced by BA in a dose dependent manner at concentration of 0.625 and 10 g/mL. The production of NO associated with iNOS was inhibited when treated with LPS at the concentration of 2.5 to 20 g/mL of BA whereas COX-2 expression was decreased at 2.5 to 20 g/mL. These modulations of inflammatory mediators were examined in LPS-stimulated RAW 264.7 cells and peritoneal macrophages. The morphology of macrophage was also examined and enhanced surface CD 40 molecule was expressed when treated BA at 0.625∼5 g/mL with or without LPS. Furthermore, BA (20 g/mL) enhanced apoptosis by producing DNA ladder in the RAW 264.7 cells. Our results indicated that BA induced activation of macrophage and pro-inflammatory cytokines. This may provide a molecular basis for the ability of BA to mediate macrophage, suppress inflammation, and modulate the immune response.
|
|
|
KEYWORD
|
|
|
Lycopus lucidus, Betulinic acid, Macrophage
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|